TITLE:
A Validated High-Performance Thin-Layer Chromatography (Hptlc) Method for the Quantitative Determination of Tricin in Two Spergularia Species
AUTHORS:
Omnia Gamal El-Dien, Eman Shawky, Amal H. Aly, Rokia M. Abdallah, Nabil A. Abdel-Salam
KEYWORDS:
Spergularia marina; Spergularia diandra; HPTLC; Quantitative; Tricin
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.11,
November
27,
2013
ABSTRACT: A simple, sensitive, precise and robust high-performance
thin-layer chromatographic (HPTLC) method for analysis of tricin has been
developed and validated for the determination of tricin in genus Spergularia. Analysis of tricin was performed
on pre-coated TLC aluminum plates with silica gel as the stationary phase. Linear
ascending development was carried out in double twin trough glass chamber
saturated with a mobile phase consisting of chloroform: methanol (37:3 v/v).
Spectro-densitometric scanning was performed by TLC scanner III (CAMAG) in
absorbance mode at the wavelength of 270 nm. The system was
found to give compact spots for tricin (Rf value of 0.34 ± 0.04). The linear
regression analysis data for the calibration plots showed a good
linear relationship with r2 = 0.9981 in the concentration range 2-15 μl
with respect to peak area. According to the International Conference on
Harmonization (ICH) guidelines, the method was validated for precision,
recovery, and robustness. The tricin content quantified from Spergularia marina and S. daiandra was found to
be 0.398 mg/100g and 1.642 mg/100g fresh weight, respectively. Statistical
analysis of the data showed that the method is reproducible and selective for
the estimation of tricin.