TITLE:
Effect of Maltose Concentration on Plant Regeneration of Anther Culture with Different Genotypes in Rice (Oryza sativa L.)
AUTHORS:
Seul Gi Park, Mohammad Ubaidillah, Kyung-Min Kim
KEYWORDS:
Anther Culture; Maltose; Oryza sativa; Plant Regeneration; Rice
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.11,
November
22,
2013
ABSTRACT: This study
describes the impact of different concentrations of maltose on plant
regeneration of anther culture for five genotypes of rice (Oryza sativa). N6 medium
was used for calli induction, while N6 medium supplemented with
different concentrations of maltose, 2.0 mg/L NAA and 0.5 mg/L kinase was used
for plant regeneration. The result showed that during the initial
stages of calli induction the anther cultures had varying rates of calli
formation among genotypes, with the best frequency being observed for Dreami2/CaMsrB2-8-DH-1
with a calli frequency of 27.8%. Different genotypes of rice cultured
in regeneration media showed varying plantlet regeneration on
media supplemented with different concentrations of maltose, with low
concentrations (0.04 g/L) leading to low frequency regeneration plantlet but
high green plant production. Indeed, when Dreami2/CaMsrB2-8-DH-2 and
Dreami2/CaMsrB2-8-DH-5 were cultivated under these conditions, 100% green
plants were observed. Another genotype also showed a small rate of
albino frequency in response to the lowest concentration of maltose, while
increased maltose concentrations resulted in increased rates of albino
plants. Overall, the results of this study should facilitate establishment
of an efficient plant regeneration system from anther culture in rice.