TITLE:
Assessment of Polyamines and Trehalose in Wheat Microspores Culture for Embryogenesis and Green Regenerated Plants
AUTHORS:
Amina Redha, Patrice Suleman
KEYWORDS:
Androgenesis; Microspore Culture; Polyamines; Trehalose; Triticum Aestivum
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.11,
November
14,
2013
ABSTRACT: Most aspects of microspore culture protocol have the capacity to cause
stress to microspores, hence, less stressful treatments might be required to avoid deleterious
effects. In stressed plants, polyamines and trehalose can act as compatible solutes or osmoprotectants by
stabilizing proteins and biological membranes. To improve green plant regeneration
in wheat microspore culture, this study assessed the effects of polyamines
(putrecine, spermidine, spermine) and trehalose on androgenic response namely
embryogenesis, green plant regeneration and ploidy of green plants regenerated
in three spring wheat genotypes. Microspores of the genotypes produced
significant numbers of embryos and green plants among polyamine treatments but
trehalose had no effect (P ≤ 0.05). Polyamine treatments for 30 min generally
produced more green plants per 100 microspores than the 60 min treatments in
all three genotypes. At least three out of twelve polyamine treatments in each
genotype improved the production of double haploid plants and seed setting in
regenerants. Wheat genotype, concentration and duration of polyamine treatment
had significant impact on embryogenesis and regeneration of green plants in
this study. The study also showed that polyamines could be used to accelerate cultivar development in wheat
breeding.