Dr. Dashuang Shi

George Washington University, USA

Principle Investigator

Email: [email protected]

Qualifications

1997 Ph.D., University of Sydney, Chemistry

1987 M.S., Xiamen University, Chemistry

1983 B.S., Xiamen University, Chemistry

Publications (selected)

1. Nguyen B, Ma R, Tang W K, et al. Crystal structure of P. falciparum Cpn60 bound to ATP reveals an open dynamic conformation before substrate binding[J]. Scientific Reports, 2021, 11(1): 5930.

2. Nguyen B, Ma R, Tang W K, et al. Crystal structure of P. falciparum Cpn60 bound to ATP reveals an open dynamic conformation before su[J]. Sci. Rep., 2020, 11.
3. Shi D, Caldovic L, Tuchman M. Sources and fates of carbamyl phosphate: a labile energy-rich molecule with multiple facets[J]. Biology, 2018, 7(2): 34.
4. Shi D, Zhao G, Mew N A, et al. Precision medicine in rare disease: mechanisms of disparate effects of N-carbamyl-l-glutamate on mutant CPS1 enzymes[J]. Molecular genetics and metabolism, 2017, 120(3): 198-206.
5. Shi D, Zhao G, Mew N A, et al. N-CARBAMYL-L-GLUTAMATE IS A CHAPERON FOR CPS1 E1034G MUTANT: CORRELATION WITH AUGMENTED UREAGENESIS AND CLINICAL IMPROVEMENT[C]//MOLECULAR GENETICS AND METABOLISM. 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2016, 117(3): 286-286.
6. Shi D, Allewell N M, Tuchman M. From genome to structure and back again: a family portrait of the transcarbamylases[J]. International journal of molecular sciences, 2015, 16(8): 18836-18864.
7. Shi D, Allewell N M, Tuchman M. The N-acetylglutamate synthase family: structures, function and mechanisms[J]. International Journal of Molecular Sciences, 2015, 16(6): 13004-13022.
8. Wu, J., Shen, E., Shi, D., Sun, Z. and Cai, T. (2012) Identification of a novel Cys146X mutation of SOD1 in familial amyotrophic lateral sclerosis by whole-exome sequencing. Genetic in Medicine, 14, in press.
9. Wei, J., Xue, Y., Wu, L., Ma, J., Yi, X., Zhang, J., Lu, B., Li, C., Shi, D., Shi, S., Feng, X. and Cai, T. (2012) Analysis of large phenotypic cariability of EEC and SHFM4 syndromes caused by K193E mutation of the TP63 gene. Plos One, 7:e35337.
10. Shi, D., Yu, X., Zhao, G., Ho, J., Lu, S., Allewell, N.M. and Tuchman, M. (2012) Crystal structure and biochemical properties of putrescine carbamoyltransferase from Enterococcus faecalis: assembly, active site and allosteric regulation. Proteins, 80, 1436-1447.
11. Shi, D., Li, Y., Cabrera-Luque, J., Jin, Z., Yu, X., Zhao, G., Haskins, N., Allewell, N.M. and Tuchman, M. (2011) A Novel N-acetylglutamate synthase architecture revealed by the crystal structure of the bifunctional enzyme from Maricaulis maris. PLoS One, 6:e28825.
12. Li, Y., Jin, Z., Yu, X., Allewell, N.M., Tuchman, M. and Shi, D. (2011) The ygeW encoded protein from Escherichia coli is a knotted ancestral catabolic transcarbamylase, Proteins, 79, 2327-2334.
13. Li, Y., Yu, X., Ho, J., Fushman, D., Allewell, N.M., Tuchman, M. and Shi, D. (2010) Reversible post-translational carboxylation modulates the enzymatic activity of N-acetyl-L-ornithine transcarbamylase. Biochemistry, 49, 6887-6895.
14. Caldovic, L., Mew, N.A., Shi, D., Morizono, H., Yudkoff, M., Tuchman, M. (2010) N-acetylglutamate synthase: structure, function and defects. Molecular Genetics and Metabolism, 199, S13-S19.
15. Ou, W., King, H., Delisle, J., Shi, D., Wilson, C.A. (2009) Phenylalanines at positions 88 and 159 of Ebolavirus envelope glycoprotein differentially impact envelope function. Virology, 396, 135-142.
16. Min, Li., Jin, Z., Caldovic, L., Morizono, H., Allewell, N.M., Tuchman, M. and Shi, D. (2009) Mechanism of allosteric inhibition of N-acetyl-glutamate synthase by L-arginine. J. Biol. Chem. 284, 4873-4880.
17. Shi, D., Sagar, V., Jin, Z., Yu, X., Caldovic, L., Morizono, H., Allewell, N. M. and Tuchman, M. (2008) The Crystal Structure of N-Acetyl-L-glutamate Synthase from Neisseria gonorrhoeae Provides Insights into Mechanisms of Catalysis and Regulation. J. Biol. Chem. 283, 7176-7184.
18. Shi, D., Yu, X., Cabrera-Luque, J., Chen, T., Roth, L., Morizono, H., Allwell, N. M. and Tuchman, M. (2007) A single mutation in the active site swaps the substrate specificity of N-acetyl-L-ornithine transcarbamylase and N-succinyl-L-ornithine transcarbamylase. Protein Sci. 16, 1689-1699.
19. Qu, Q., Morizono, H., Shi, D., Tuchman, M. and Caldovic, L. (2007) A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N-acetylglutamate synthase. BMC Biochem. 8, 4.
20. Shi, D., Yu, X., Roth, L., Tuchman, M. and Allewell, N.M. (2007) Structure of A Novel N-Acetyl-L-citrulline deacetylase from Xanthomonas campestris. Biophys. Chem. 126, 86-93.

Profile Details

https://scholar.google.com/citations?user=mL6VFkIAAAAJ&hl=en

https://www.researchgate.net/profile/Dashuang-Shi

WoS Researcher ID: DTZ-3451-2022